7U30
PRMT5:MEP50 Complexed with Cyclonucleoside Compound 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.116 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 104.418, 138.742, 178.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.350 - 2.600 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oop |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.870 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.160 | 2.710 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.065 | 1.296 |
| Number of reflections | 40073 | 4451 |
| <I/σ(I)> | 26.2 | 2.2 |
| Completeness [%] | 99.5 | |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M sodium citrate pH 6.0, 0.2 M sodium acetate, 10-12% PEG 4000 |
