7U30

PRMT5:MEP50 Complexed with Cyclonucleoside Compound 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.116
Spacegroup name	I 2 2 2
Unit cell lengths	104.418, 138.742, 178.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.350 - 2.600
R-factor	0.21
Rwork	0.210
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oop
RMSD bond length	0.010
RMSD bond angle	0.870
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	REFMAC
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.160	2.710
High resolution limit [Å]	2.600	2.600
Rmerge	0.065	1.296
Number of reflections	40073	4451
<I/σ(I)>	26.2	2.2
Completeness [%]	99.5
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1 M sodium citrate pH 6.0, 0.2 M sodium acetate, 10-12% PEG 4000