7U2J

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, peptidyl P-site fMAC-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.930, 451.240, 622.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	125.000 - 2.550
R-factor	0.2157
Rwork	0.213
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.000	125.000	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.167	0.070	2.281
Rmeas	0.183	0.076	2.485
Total number of observations	11553376
Number of reflections	1882520	21658	139410
<I/σ(I)>	7.9	24.75	0.89
Completeness [%]	99.5	97	100
Redundancy	6.137	6.193	6.285
CC(1/2)	0.993	0.993	0.171

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME