7U2J

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, peptidyl P-site fMAC-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution

This is a non-PDB format compatible entry.

Summary for 7U2J

• Entry DOI: 10.2210/pdb7u2j/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (63 entities in total)
• Functional Keywords: chloramphenicol; antibiotic; glycine; non-hydrolyzable; aminoacyl-trna; peptidyl-trna; 70s ribosome; x-ray structure; pre-attack state; transpeptidation; peptidyl transferase center; context-specificity of drug action; inhibition of translation; uncompetitive inhibitor, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 114
• Total formula weight: 4564913.12

Authors

Syroegin, E.A.,Aleksandrova, E.V.,Polikanov, Y.S. (deposition date: 2022-02-24, release date: 2022-07-13, Last modification date: 2023-11-15)

• Primary citation: Syroegin, E.A.,Aleksandrova, E.V.,Polikanov, Y.S.; Structural basis for the inability of chloramphenicol to inhibit peptide bond formation in the presence of A-site glycine.; Nucleic Acids Res., 50:7669-7679, 2022; PubMed: 35766409; DOI: 10.1093/nar/gkac548

Experimental method

X-RAY DIFFRACTION (2.55 Å)