7U20

Crystal structure of human METTL1 and WDR4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	124.407, 194.574, 86.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.870 - 3.100
R-factor	0.224
Rwork	0.222
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3CKK for METTL1 chain D of 2VDU for WDR4
RMSD bond length	0.002
RMSD bond angle	0.435
Data reduction software	XDS (VERSION Jan 31, 2020 BUILT=20200131)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.870	47.870	3.310
High resolution limit [Å]	3.090	8.750	3.090
Rmerge	0.308	0.039	2.649
Rmeas	0.377	0.048	3.236
Rpim	0.214	0.028	1.831
Number of reflections	19262	892	3381
<I/σ(I)>	5.1	20.8	0.9
Completeness [%]	99.0	96.4	97.5
Redundancy	4.6	4.5	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	2M (NH4)2SO4, 0.2M K Na Tartrate, 0.1 M Na3 citrate pH 6.5