7U0K

IOMA class antibody Fab ACS124

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	62.452, 66.703, 101.939
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.980 - 1.730
R-factor	0.1872
Rwork	0.185
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f7e
RMSD bond length	0.011
RMSD bond angle	1.149
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.980	1.740
High resolution limit [Å]	1.730	1.730
Rmerge	0.155	1.790
Rmeas	0.162	1.870
Rpim	0.046	0.526
Number of reflections	46469	2300
<I/σ(I)>	36.3	1.3
Completeness [%]	98.9	99.7
Redundancy	12	11.9
CC(1/2)	0.890	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M of CHES (pH 9.5) and 38% (v/v) polyethylene glycol 600