7TXW

Crystal structure of the complex of the malaria sexual stage protein and vaccine target Pfs25 with the Fab fragment of a transmission blocking antibody 1G2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	95
Detector technology	CCD
Collection date	2016-07-05
Detector	MAR CCD 130 mm
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	122.320, 50.170, 100.030
Unit cell angles	90.00, 123.10, 90.00

Refinement procedure

Resolution	60.970 - 2.170
R-factor	0.1804
Rwork	0.177
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house Fab models
RMSD bond length	0.009
RMSD bond angle	1.197
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.970	2.230
High resolution limit [Å]	2.170	2.170
Rmerge	0.068	0.318
Number of reflections	26678	1649
<I/σ(I)>	18.62	4.22
Completeness [%]	98.3	83.6
Redundancy	4.92	3.46
CC(1/2)		0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293.15	20-25% PEG 6000, 0.1 M HEPES, pH 7.5 and cocktail of low molecular alcohols as additives