7TW9
Structure of nsp14 N7-MethylTransferase domain fused with TELSAM bound to Sinefungin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.92010 |
Spacegroup name | P 65 |
Unit cell lengths | 109.028, 109.028, 48.641 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.290 - 1.410 |
R-factor | 0.1943 |
Rwork | 0.192 |
R-free | 0.23190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c8t 7n1o |
RMSD bond length | 0.008 |
RMSD bond angle | 0.794 |
Data reduction software | DIALS (2.2.10) |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20rc3_4406) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.520 | 1.600 |
High resolution limit [Å] | 1.410 | 1.410 |
Rmerge | 0.108 | 1.190 |
Rmeas | 0.112 | |
Rpim | 0.049 | 0.729 |
Number of reflections | 47235 | 1275 |
<I/σ(I)> | 9.2 | |
Completeness [%] | 90.0 | 71.3 |
Redundancy | 11.1 | 7.1 |
CC(1/2) | 0.996 | 0.493 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 11-17% Reagent Alcohol, 0.1-0.3M Lithium Sulfate, and 0.1M Sodium Citrate pH 5.5 |