7TW8

Structure of nsp14 N7-MethylTransferase domain fused with TELSAM bound to SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	P 65
Unit cell lengths	109.147, 109.147, 48.654
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.320 - 1.550
R-factor	0.1774
Rwork	0.176
R-free	0.20560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c8t 7n1o
RMSD bond length	0.004
RMSD bond angle	0.831
Data reduction software	DIALS (2.2.10)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.520	1.680
High resolution limit [Å]	1.550	1.550
Rmerge	0.068
Rmeas	0.085
Rpim	0.031
Number of reflections	58525	294
<I/σ(I)>	13.4
Completeness [%]	95.4	71.6
Redundancy	10.6	5.9
CC(1/2)	0.999	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	11-17% Reagent Alcohol, 0.1-0.3M Lithium Sulfate and 0.1M Sodium Citrate pH 5.5