7TW7

Structure of nsp14 N7-MethylTransferase domain fused with TELSAM bound to SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	P 65
Unit cell lengths	108.774, 108.774, 48.527
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.210 - 1.620
R-factor	0.1808
Rwork	0.179
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c8t 7n1o
RMSD bond length	0.010
RMSD bond angle	1.168
Data reduction software	DIALS (2.2.10)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.200	1.770
High resolution limit [Å]	1.620	1.620
Rmerge	0.070	0.880
Rpim	0.039	0.560
Number of reflections	47235	1275
<I/σ(I)>	12.4	2
Completeness [%]	90.0	76
Redundancy	8.1	5.8
CC(1/2)	0.998	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	11-17% Reagent Alcohol, 0.1-0.3M Lithium Sulfate, and 0.1M Sodium Citrate pH 5.5