7TVD
Crystal structure of the kinase domain of EGFR exon-19 (del-747-749) mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 149.420, 149.420, 149.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.710 - 2.960 |
| R-factor | 0.2422 |
| Rwork | 0.240 |
| R-free | 0.27740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m17 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.766 |
| Data reduction software | XDS (20200417) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 105.660 | 105.660 | 3.140 |
| High resolution limit [Å] | 2.960 | 8.880 | 2.960 |
| Rmerge | 0.259 | 0.055 | 6.596 |
| Rmeas | 0.263 | 0.056 | 6.690 |
| Rpim | 0.044 | 0.010 | 1.110 |
| Total number of observations | 423785 | 15923 | 67842 |
| Number of reflections | 11749 | 476 | 1880 |
| <I/σ(I)> | 15.4 | 67.6 | 0.8 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 36.1 | 33.5 | 36.1 |
| CC(1/2) | 0.999 | 1.000 | 0.438 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289.15 | 28% PEG 400, 0.1 M Na Hepes pH 7.5, 0.2 M Calcium Chloride |
