7TUO
Crystal structure analysis of human USP28 complex with a compound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 3 |
Unit cell lengths | 114.640, 114.640, 114.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.060 - 1.960 |
R-factor | 0.1791 |
Rwork | 0.178 |
R-free | 0.20310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6heh |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 81.060 | 81.130 | 1.990 |
High resolution limit [Å] | 1.960 | 5.320 | 1.960 |
Rmeas | 0.112 | 0.035 | 2.224 |
Rpim | 0.030 | 0.010 | 0.594 |
Total number of observations | 496809 | 24197 | 25005 |
Number of reflections | 36300 | 1942 | 1792 |
<I/σ(I)> | 17.3 | 55.5 | 1.4 |
Completeness [%] | 100.0 | 100 | 99.9 |
Redundancy | 13.7 | 12.5 | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.4M Sodium maleic acid, pH 8.0 |