7TT8

Human LRH-1 LBD bound to agonist 6N-10CA and fragment of Tif2 coactivator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	88.302, 88.302, 105.605
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.200 - 2.800
R-factor	0.2337
Rwork	0.229
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l11
RMSD bond length	0.003
RMSD bond angle	0.501
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.200	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	11868	1181
<I/σ(I)>	7.7
Completeness [%]	97.9
Redundancy	5.4
CC(1/2)	0.978	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	tri-Na citrate (4.6), tert-butanol, glycerol