7TRL
Crystal structure of human BIRC2 BIR3 domain in complex with histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-17 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.655, 34.972, 35.605 |
| Unit cell angles | 90.00, 103.87, 90.00 |
Refinement procedure
| Resolution | 24.580 - 1.740 |
| R-factor | 0.1667 |
| Rwork | 0.162 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d9t |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.393 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rpim | 0.026 | 0.293 |
| Number of reflections | 8045 | 334 |
| <I/σ(I)> | 21.8 | |
| Completeness [%] | 97.0 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | 0.1 M SPG buffer (2:7:7 molar ratio of succinic acid:sodium dihydrogen phosphate: glycine), 25% PEG 1,500 |
