7TRI
Human antibody S8V1-172 in complex with the influenza hemagglutinin head domain of A/Sydney/05/1997(H3N2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-10-31 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 94.700, 94.700, 248.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.560 - 3.600 |
R-factor | 0.2347 |
Rwork | 0.233 |
R-free | 0.26820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xpz 6mhr 6e4x |
RMSD bond length | 0.003 |
RMSD bond angle | 0.605 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.560 | 3.729 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.046 | 0.323 |
Number of reflections | 15613 | 1543 |
<I/σ(I)> | 12.72 | |
Completeness [%] | 99.5 | 99.87 |
Redundancy | 2 | 2 |
CC(1/2) | 0.996 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.1 M tris(hydroxymethyl)aminomethane pH 8.5 and 25% (w/v) poly(ethylene glycol) 3350 |