7TRH
Human antibody K03.28 in complex with the influenza hemagglutinin head domain of A/California/07/2009(H1N1)(X-181)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-08-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.830, 43.650, 96.910 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 46.800 - 3.000 |
| R-factor | 0.2038 |
| Rwork | 0.202 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ube 6e56 4hk0 4wuk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.626 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.040 | 0.382 |
| Number of reflections | 24401 | 2401 |
| <I/σ(I)> | 14.93 | |
| Completeness [%] | 98.3 | |
| Redundancy | 2 | |
| CC(1/2) | 0.998 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 25% (w/v) poly(ethylene glycol) 1500 |
