7TKW
Crystal structure of R14A human Galectin-7 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97952 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.750, 64.950, 70.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.740 - 1.850 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.046 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.7.12) |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.750 | 53.770 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.692 | 0.695 | 0.888 |
| Rmeas | 0.724 | 0.728 | 0.927 |
| Rpim | 0.208 | 0.212 | 0.264 |
| Number of reflections | 21688 | 2333 | 2125 |
| <I/σ(I)> | 142.7 | 568.1 | 12.8 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 12.1 | 11.5 | 12.1 |
| CC(1/2) | 0.711 | 0.610 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.2 M Sodium chloride, 0.1 M Tris 8.0, 20 % w/v PEG 6000 |
