7TJC

VHH Chl-B2 in complex with Chloramphenicol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	43.219, 66.553, 93.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.150 - 1.350
R-factor	0.2015
Rwork	0.200
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bzq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.150	1.400
High resolution limit [Å]	1.350	1.350
Rpim	2.400	0.398
Number of reflections	59730	6000
<I/σ(I)>	9.1	1
Completeness [%]	99.7	98.2
Redundancy	12.7	10.6
CC(1/2)	0.999	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	100 mM sodium acetate, 1 M di-ammonium hydrogen phosphate