7T4V
Crystal Structure of cGMP-dependent Protein Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.232, 103.690, 104.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.160 - 2.280 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25600 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.540 | 2.603 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.221 | 0.941 |
| Rpim | 0.100 | 0.504 |
| Number of reflections | 22622 | 1132 |
| <I/σ(I)> | 6.5 | 1.5 |
| Completeness [%] | 92.9 | 68.4 |
| Redundancy | 5.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 289 | 0.1M SPG pH 8.0 25% PEG1500 |
