7T2M

Crystal Structure of TEAD2 in a covalent complex with TED-664

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2021-04-15
Detector	RDI CMOS_8M
Wavelength(s)	1.07216
Spacegroup name	C 1 2 1
Unit cell lengths	111.106, 61.570, 79.511
Unit cell angles	90.00, 112.69, 90.00

Refinement procedure

Resolution	52.780 - 2.810
R-factor	0.2242
Rwork	0.221
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e5g
RMSD bond length	0.010
RMSD bond angle	1.067
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.780	3.110
High resolution limit [Å]	2.810	2.810
Rmeas	0.163	1.077
Rpim	0.084	0.560
Number of reflections	7956	398
<I/σ(I)>	5.7	1.2
Completeness [%]	89.9	59.8
Redundancy	3.7	3.7
CC(1/2)	0.993	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium Formate 1.8M - 2.4M HEPES pH 7.2 - 7.4