7T1Z
Structure of the Fbw7-Skp1-MycNdegron complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 123 |
Detector technology | CCD |
Collection date | 2021-04-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 232.869, 232.869, 107.161 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.960 - 2.770 |
R-factor | 0.2244 |
Rwork | 0.223 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ovp |
RMSD bond length | 0.026 |
RMSD bond angle | 1.404 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.820 |
High resolution limit [Å] | 2.770 | 2.770 |
Number of reflections | 37654 | 1861 |
<I/σ(I)> | 12 | |
Completeness [%] | 100.0 | |
Redundancy | 9.7 | |
CC(1/2) | 0.992 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298.15 | 0.1 M Bicine pH6.5, 1.6 M Li2SO4 |