7T18
Rev1 Ternary Complex with dTTP and Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-09 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.202, 73.639, 117.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.660 - 1.700 |
| R-factor | 0.1838 |
| Rwork | 0.182 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wm1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.991 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.660 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 60303 | 2977 |
| <I/σ(I)> | 15.06 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.999 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15-23% PEG3350, 200 mM ammonium nitrate |
