7T0Y

The Ribosomal RNA Processing 1B Protein Phosphatase-1 Holoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	83.528, 160.387, 50.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.270 - 1.800
R-factor	0.2093
Rwork	0.208
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mov
RMSD bond length	0.005
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.19.1_4158)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.270	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.207	1.280
Number of reflections	61295	4407
<I/σ(I)>	4.9
Completeness [%]	96.2	94.8
Redundancy	4.6	4.6
CC(1/2)	0.981	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	20% ethylene glycol; 10% PEG8000, 0.1 M imidazole; MES acid, 0.09 M Sodium fluoride; 0.09 M Sodium bromide; 0.09 M Sodium iodide