7SUE
Crystal Structure of Human Fab S24-188 in the complex with the N-teminal Domain of Nucleocapsid protein from SARS CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-03 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.310, 78.500, 127.150 |
| Unit cell angles | 97.60, 89.96, 90.02 |
Refinement procedure
| Resolution | 39.200 - 2.900 |
| R-factor | 0.264 |
| Rwork | 0.262 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | swiss model |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.635 |
| Data reduction software | xia2 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.085 | 1.428 |
| Number of reflections | 55485 | 2773 |
| <I/σ(I)> | 8.6 | 0.9 |
| Completeness [%] | 91.6 | 92.2 |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.997 | 0.481 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1 M Sodium Citrate pH 5.6, 20 % (w/v) PEG 4000, 20 % (v/v) 2-propanol |
