7SSE

Crystal structure of the WDR domain of human DCAF1 in complex with CYCA-117-70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	48.956, 87.919, 73.878
Unit cell angles	90.00, 98.72, 90.00

Refinement procedure

Resolution	44.000 - 1.620
R-factor	0.2069
Rwork	0.206
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pxw
RMSD bond length	0.005
RMSD bond angle	1.307
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.650
High resolution limit [Å]	1.620	4.400	1.620
Rmerge	0.041	0.030	0.516
Rmeas	0.048	0.035	0.651
Rpim	0.024	0.018	0.390
Total number of observations	269845
Number of reflections	76489	3953	3068
<I/σ(I)>	13.5
Completeness [%]	97.4	98.4	78.4
Redundancy	3.5	3.7	2.1
CC(1/2)		0.997	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	30% PEG3350 ,0.1M Ammonium Sulphate, 0.1M Bis-Tris pH5.5