7SPT

Crystal structure of exofacial state human glucose transporter GLUT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-10-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	48.231, 118.430, 53.728
Unit cell angles	90.00, 103.16, 90.00

Refinement procedure

Resolution	46.965 - 2.100
R-factor	0.1788
Rwork	0.177
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zw9
RMSD bond length	0.007
RMSD bond angle	0.786
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (dev_2405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	34213	4424
<I/σ(I)>	6.34
Completeness [%]	99.9
Redundancy	13.49
CC(1/2)	0.980	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	40% v/v PEG400, 100 mM Tris, pH 7.6, 100 mM magnesium chloride