7SPS

Crystal structure of human glucose transporter GLUT3 bound with exofacial inhibitor SA47

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-10-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	77.591, 121.552, 95.872
Unit cell angles	90.00, 108.14, 90.00

Refinement procedure

Resolution	46.390 - 2.300
R-factor	0.213
Rwork	0.212
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zw9
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	75008	8977
<I/σ(I)>	11.75
Completeness [%]	99.9
Redundancy	23.07
CC(1/2)	0.998	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	40% v/v PEG400, 100 mM sodium citrate, pH 5.7, 400 mM potassium formate