7SOR
LaM domain of human LARP1 in complex with AAA RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-09 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.96860 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.562, 55.890, 70.172 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.720 - 1.350 |
R-factor | 0.1819 |
Rwork | 0.181 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7soo |
RMSD bond length | 0.014 |
RMSD bond angle | 1.584 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 42380 | 4097 |
<I/σ(I)> | 44.5 | |
Completeness [%] | 99.8 | |
Redundancy | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M sodium chloride, 0.1M Bis-Tris pH 5.5, 25% PEG3350 |