7SNX

1.70A Resolution Structure of NanoBiT Complementation Reporter Complex of LgBit and SmBiT Subunits

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	73.418, 73.418, 101.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.710 - 1.700
R-factor	0.1578
Rwork	0.157
R-free	0.17850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ibo
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (dev_4289)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.180	46.180	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.116	0.033	1.307
Total number of observations	214426	1590	11527
Number of reflections	31166	273	1627
<I/σ(I)>	12.2	37.3	1.6
Completeness [%]	100.0	99.5	100
Redundancy	6.9	5.8	7.1
CC(1/2)	0.998	0.999	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	1.5 M Na/K Phosphate, 4 (v/v) % 1,3-propanediol