7SN6

U2AF65 UHM BOUND TO SF3B155 ULM5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	76.163, 63.156, 49.387
Unit cell angles	90.00, 94.37, 90.00

Refinement procedure

Resolution	37.970 - 1.800
R-factor	0.1834
Rwork	0.180
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fxw
Data reduction software	XDS (November 3, 2014)
Data scaling software	Aimless (0.5.8)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.970	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.038	0.499
Rpim	0.023	0.304
Number of reflections	21337	1256
<I/σ(I)>	16.7	3
Completeness [%]	98.4
Redundancy	3.6	3.6
CC(1/2)	0.990	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 5.6, 15% V/V 2-PROPANOL, 20% PEG 4000