7SLS
HIV Reverse Transcriptase with compound Pyr02
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-05 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.728, 154.253, 155.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.580 - 2.078 |
| R-factor | 0.2282 |
| Rwork | 0.227 |
| R-free | 0.25850 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.8 (10-DEC-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.586 | 2.224 |
| High resolution limit [Å] | 2.078 | 2.078 |
| Number of reflections | 69946 | 3498 |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 82.2 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.627 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 950 mM sodium malonate pH 7.0, 100 mM HEPES/NaOH pH 6.8 |
