7SIT
Crystal structure of Voltage gated potassium ion channel, Kv 1.2 chimera-3m
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2020-03-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 129.587, 129.587, 278.482 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.159 - 3.320 |
R-factor | 0.2529 |
Rwork | 0.251 |
R-free | 0.28930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r9r |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.160 | 3.510 |
High resolution limit [Å] | 3.320 | 3.320 |
Number of reflections | 35720 | 4588 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 99.9 | |
Redundancy | 14.9 | |
CC(1/2) | 0.990 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 50 mM Tris, pH 8.5, 28-32% PEG400, CHAPS |