7SHY
IgE-Fc in complex with omalizumab scFv
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979460 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.500, 179.090, 91.520 |
Unit cell angles | 90.00, 111.66, 90.00 |
Refinement procedure
Resolution | 39.930 - 3.000 |
Rwork | 0.173 |
R-free | 0.22990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hys |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.930 | 3.107 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 160286 | 16540 |
<I/σ(I)> | 11.78 | 1.29 |
Completeness [%] | 98.0 | |
Redundancy | 3.5 | |
CC(1/2) | 0.998 | 0.635 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.02 M Magnesium chloride, 0.1M HEPES pH 7.5, 22% (w/v) Polyacrylic acid 5100 sodium salt |