7SHY

IgE-Fc in complex with omalizumab scFv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979460
Spacegroup name	C 1 2 1
Unit cell lengths	157.500, 179.090, 91.520
Unit cell angles	90.00, 111.66, 90.00

Refinement procedure

Resolution	39.930 - 3.000
Rwork	0.173
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hys
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.930	3.107
High resolution limit [Å]	3.000	3.000
Number of reflections	160286	16540
<I/σ(I)>	11.78	1.29
Completeness [%]	98.0
Redundancy	3.5
CC(1/2)	0.998	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.02 M Magnesium chloride, 0.1M HEPES pH 7.5, 22% (w/v) Polyacrylic acid 5100 sodium salt