7SHY

IgE-Fc in complex with omalizumab scFv

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	I, K, C, E	Omalizumab VH	polymer	122	13362.7	4		Homo sapiens
2	J, L, D, F	Omalizumab VL	polymer	134	14665.8	4		Homo sapiens
3	A, G, H, B	IgE Fc	polymer	247	27550.9	4	UniProt (P01854) Pfam (PF07654) In PDB	Homo sapiens (Human)	Immunoglobulin heavy constant epsilon, Ig epsilon chain C region,Ig epsilon chain C region ND
4	M	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(2-3)-[alpha-L-fucopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1057.0	1
5	N	beta-D-mannopyranose-(1-4)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1
6	O	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1
7	P	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1
8	D	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
9	A, H, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
10	H	alpha-D-mannopyranose	non-polymer		180.2	1	Chemie (MAN)
11		water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, G, H, B: 328 - 545 (UniProt: P01854)

PDB	External Database	Details
Ala 299	-	expression tag
Pro 300	-	expression tag
Met 301	-	expression tag
Ala 302	-	expression tag
Glu 303	-	expression tag
Gly 304	-	expression tag
Gly 305	-	expression tag
Gly 306	-	expression tag
Gln 307	-	expression tag
Asn 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
Gly 317	-	expression tag
Gly 318	-	expression tag
Glu 319	-	expression tag
Asn 320	-	expression tag
Leu 321	-	expression tag
Tyr 322	-	expression tag
Phe 323	-	expression tag
Gln 324	-	expression tag
Gly 325	-	expression tag
Gly 326	-	expression tag
Ser 327	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	222317.8
Branched	Number of molecules	4
	Total formula weight	4113.7
Non-Polymers*	Number of molecules	5
	Total formula weight	935.9
All*	Total formula weight	227367.4

*Water molecules are not included.