7SHU

IgE-Fc in complex with omalizumab variant C02

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.978720
Spacegroup name	C 2 2 21
Unit cell lengths	90.070, 176.120, 142.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.190 - 2.750
R-factor	0.2226
Rwork	0.220
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hys
RMSD bond length	0.003
RMSD bond angle	0.548
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.190	2.848
High resolution limit [Å]	2.750	2.750
Number of reflections	29871	2928
<I/σ(I)>	19.21	1.83
Completeness [%]	99.8
Redundancy	8.3
CC(1/2)	0.999	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M Lithium sulfate, 0.1 M Phosphate citrate pH 4.2, 20% (w/v) PEG 1000