7SEG

Crystal structure of the complex of CD16A bound by an anti-CD16A Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-04-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	45.307, 220.560, 70.825
Unit cell angles	90.00, 108.55, 90.00

Refinement procedure

Resolution	33.885 - 2.156
R-factor	0.1938
Rwork	0.189
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	14EJ
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.190
High resolution limit [Å]	2.150	5.830	2.150
Rmerge	0.098	0.068	0.552
Rmeas	0.119	0.081	0.734
Rpim	0.065	0.044	0.479
Total number of observations	184828
Number of reflections	64779	3475	2209
<I/σ(I)>	6.1
Completeness [%]	92.8	98.2	64.1
Redundancy	2.9	3.4	1.7
CC(1/2)		0.991	0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.1M Bis-Tris pH5.5, 0.2M MgCl2, 0.01M Betaine HCl, 25% PEG3350