7SCX
KRAS full-length G12V in complex with RGL1 Ras association domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.950, 38.770, 68.240 |
| Unit cell angles | 90.00, 116.72, 90.00 |
Refinement procedure
| Resolution | 33.860 - 1.960 |
| R-factor | 0.1755 |
| Rwork | 0.173 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lfd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.860 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.023 | 0.344 |
| Rmeas | 0.032 | 0.487 |
| Rpim | 0.023 | 0.344 |
| Number of reflections | 20768 | 2049 |
| <I/σ(I)> | 15.1 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 4 |
| CC(1/2) | 0.999 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1M BIS-TRIS (pH 5.5), 25% w/v PEG 3350 |
