7SCX

KRAS full-length G12V in complex with RGL1 Ras association domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	121.950, 38.770, 68.240
Unit cell angles	90.00, 116.72, 90.00

Refinement procedure

Resolution	33.860 - 1.960
R-factor	0.1755
Rwork	0.173
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lfd
RMSD bond length	0.013
RMSD bond angle	1.158
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.860	2.030
High resolution limit [Å]	1.960	1.960
Rmerge	0.023	0.344
Rmeas	0.032	0.487
Rpim	0.023	0.344
Number of reflections	20768	2049
<I/σ(I)>	15.1	2.3
Completeness [%]	99.9	100
Redundancy	4	4
CC(1/2)	0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.1M BIS-TRIS (pH 5.5), 25% w/v PEG 3350