7SCF

M. tb EgtD in complex with HD2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	69.102, 71.570, 145.095
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.950 - 2.670
R-factor	0.2193
Rwork	0.213
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uy5
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.950	2.670
High resolution limit [Å]	2.670	2.670
Rmerge	0.100	0.450
Number of reflections	21089	2068
<I/σ(I)>	10.89
Completeness [%]	99.8
Redundancy	1
CC(1/2)	0.960	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.2 M potassium phosphate dibasic and 20 % w/v polyethylene glycol 3,350