7SC4
filamin complex-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 50.248, 66.010, 71.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.970 - 1.850 |
| R-factor | 0.2019 |
| Rwork | 0.199 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2brq |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.757 |
| Data reduction software | HKL-3000 (v1.0) |
| Data scaling software | HKL-3000 (v1.0) |
| Phasing software | PHASER (v1.0) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 1.800 | 2.200 |
| Rmerge | 0.110 | 0.489 |
| Number of reflections | 55864 | 604 |
| <I/σ(I)> | 20 | 3 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 5.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 2 M Ammonium Sulfate, 5% 2-propanol |
