7SA7

Crystal structure of the apo SH2 domains of Syk

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999816
Spacegroup name	C 1 2 1
Unit cell lengths	143.882, 153.881, 85.472
Unit cell angles	90.00, 91.05, 90.00

Refinement procedure

Resolution	45.780 - 3.200
R-factor	0.2711
Rwork	0.270
R-free	0.30390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a81
RMSD bond length	0.002
RMSD bond angle	0.661
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.780	3.314
High resolution limit [Å]	3.200	3.200
Rmerge	0.323	2.097
Number of reflections	30660	3029
<I/σ(I)>	7.47
Completeness [%]	99.7
Redundancy	8.3
CC(1/2)	0.988	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Protein- 35mg/mL in 10mM HEPES pH 7.0, 50mM NaCl, 5% glycerol and 1mM TCEP Crystal conditions-0.2M sodium nitrate and 10% w/v PEG 3350 Cryoprotectant-0.2M sodium nitrate, 20% PEG 3350, and 20% glycerol