7S9I
Room-temperature Human Hsp90a-NTD bound to EC144
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 278 |
| Detector technology | PIXEL |
| Collection date | 2020-09-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.315, 91.151, 99.586 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.810 - 1.750 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yer |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.117 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.810 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.074 | 0.743 |
| Number of reflections | 30975 | 3036 |
| <I/σ(I)> | 9.35 | |
| Completeness [%] | 94.3 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.994 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.8 M Malic acid, pH 7 |
