7S8Y

Cryogenic apo Human Hsp90a-NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	65.146, 88.394, 99.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.200 - 1.590
R-factor	0.1659
Rwork	0.165
R-free	0.19130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yer
RMSD bond length	0.010
RMSD bond angle	1.049
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19rc5_4047)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.200	1.650
High resolution limit [Å]	1.590	1.590
Rmerge	0.093	0.678
Number of reflections	38596	3808
<I/σ(I)>	6.77
Completeness [%]	99.0
Redundancy	4.4
CC(1/2)	0.996	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100 mM HEPES, pH 7 and 1.6 M sodium citrate dihydrate