7S7K

Crystal structure of the EphB2 extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	73.843, 111.142, 156.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.400 - 3.150
R-factor	0.2153
Rwork	0.214
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fl7
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.400	48.400	3.320
High resolution limit [Å]	3.145	9.950	3.145
Rmerge		0.014	0.792
Rmeas	0.057	0.018	1.040
Rpim	0.030	0.010	0.544
Total number of observations	73799
Number of reflections	22540	746	3293
<I/σ(I)>	18	42.4	1
Completeness [%]	98.0	90.9	99.3
Redundancy	3.3	3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.1 M Na Succinate, 0.1M Na Acetate pH 4.8, 5% (v/v) MPD, and 3% 1, 6-Hexanediol