7S59

Crystal structure of the tick evasin EVA-P974 complexed to a chimera made of human chemokines CCL7 and CCL8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	126.240, 62.718, 53.966
Unit cell angles	90.00, 93.93, 90.00

Refinement procedure

Resolution	42.380 - 2.390
R-factor	0.239
Rwork	0.237
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s58
RMSD bond length	0.010
RMSD bond angle	1.245
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.380	2.480
High resolution limit [Å]	2.390	2.390
Number of reflections	16549	1551
<I/σ(I)>	7.9
Completeness [%]	98.1
Redundancy	3.3
CC(1/2)	0.997	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 M TRIS 8.5 pH (Buffer) 2 M (NH4)2SO4 (Precipitant)