7S3A

Crystal structure of intact U2AF65 RRM-region bound to AdML-C5 oligonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	43.245, 63.022, 77.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.760 - 1.480
R-factor	0.1324
Rwork	0.131
R-free	0.15730
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5ev4
Data reduction software	XDS
Data scaling software	Aimless (0.5.8)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.750	1.500
High resolution limit [Å]	1.480	1.480
Rmerge	0.042	0.196
Rmeas	0.050	0.251
Rpim	0.026	0.154
Number of reflections	35972	1368
<I/σ(I)>	22	4.9
Completeness [%]	98.7	76.4
Redundancy	6.5	3.9
CC(1/2)	0.999	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	0.60 M succinic acid, 0.10 M Hepes pH 7.0, 2% Peg MME 2000