7RXQ

Crystal structure of junctophilin-2 in complex with a CaV1.1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	116.394, 30.381, 96.679
Unit cell angles	90.00, 115.96, 90.00

Refinement procedure

Resolution	38.070 - 2.030
R-factor	0.2048
Rwork	0.202
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rw4
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.340	44.350	2.070
High resolution limit [Å]	2.030	5.510	2.030
Rmerge	0.052	0.033	0.225
Rmeas	0.062	0.040	0.271
Rpim	0.034	0.022	0.149
Number of reflections	19608	1062	939
<I/σ(I)>	13.3	37.8	1.8
Completeness [%]	97.1	97.16	97.71
Redundancy	3.18	3.11	3.21
CC(1/2)	0.998	0.998	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG3350, potassium sulphate