7RWW

Crystal structure of a Zn-bound RIDC1 variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	47.791, 86.913, 49.176
Unit cell angles	90.00, 109.16, 90.00

Refinement procedure

Resolution	35.960 - 1.700
R-factor	0.1765
Rwork	0.175
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bc5
RMSD bond length	0.012
RMSD bond angle	1.124
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.850	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.121	0.809
Rmeas	0.126	0.849
Rpim	0.032	0.341
Number of reflections	40172	3987
<I/σ(I)>	11.7	3.2
Completeness [%]	99.9
Redundancy	14.8
CC(1/2)	0.996	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	45% MPD, 0.1 M HEPES, 0.2 M MgCl2