7RQP

Structure of PfCSP NPNV binding antibody L9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	I 2 2 2
Unit cell lengths	67.964, 117.670, 143.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.920 - 2.980
R-factor	0.2039
Rwork	0.199
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d01
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.520	45.520	3.160
High resolution limit [Å]	2.980	8.940	2.980
Rmerge	0.092	0.036	1.050
Rmeas	0.096	0.038	1.091
Rpim	0.027	0.012	0.295
Total number of observations	155768	5334	26112
Number of reflections	12137	502	1919
<I/σ(I)>	17.5	55.3	2.6
Completeness [%]	100.0	98.9	100
Redundancy	12.8	10.6	13.6
CC(1/2)	0.999	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris, pH 8.0, 100mM NaCl, 8% PEG 20,000