7RMA
Structure of the fourth UIM (Ubiquitin Interacting Motif) of ANKRD13D in complex with a high affinity UbV (Ubiquitin Variant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 45.770, 45.770, 84.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.360 - 2.000 |
| R-factor | 0.2119 |
| Rwork | 0.208 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UbV from PDB 6CRN |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.726 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.360 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.067 | 0.511 |
| Rmeas | 0.077 | |
| Number of reflections | 6724 | 448 |
| <I/σ(I)> | 14 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 6.8 |
| CC(1/2) | 0.998 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.1M Li2SO4, 0.1M sodium acetate pH 4.5 and 50% PEG 400. Crystals were cryoprotected in this buffer plus 20% ethylene glycol |
