7RKG
Griffithsin mutant Y28W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2019-04-10 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.708, 55.708, 183.035 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.160 - 2.200 |
| R-factor | 0.1634 |
| Rwork | 0.160 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2guc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.872 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.17.1-3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.089 | 0.214 |
| Rmeas | 0.096 | 0.239 |
| Rpim | 0.036 | 0.105 |
| Number of reflections | 17186 | 852 |
| <I/σ(I)> | 13 | 3.1 |
| Completeness [%] | 97.8 | 99.6 |
| Redundancy | 6.8 | 3.1 |
| CC(1/2) | 0.994 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M citric acid pH 1.5 2.2 M sodium chloride |
