7RK7

The complex between TIL 1383i TCR and human Class I MHC HLA-A2 with the bound Tyrosinase(369-377)(N371D) nonameric peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	114.738, 74.428, 131.058
Unit cell angles	90.00, 93.49, 90.00

Refinement procedure

Resolution	37.210 - 2.540
R-factor	0.2135
Rwork	0.211
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qdm
RMSD bond length	0.005
RMSD bond angle	0.923
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.310	2.631
High resolution limit [Å]	2.540	2.540
Number of reflections	36440	3527
<I/σ(I)>	8.27	2.07
Completeness [%]	99.5
Redundancy	1.1
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	16% PEG 3350, 2% tacsimate, 100mM Tris (pH 8.5)